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Nucleic Acid Purification
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g-TUBE (10)
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g-TUBE (10)
g-TUBE (10)
g-TUBE (10) from Covaris LLC
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Citations:
(73)
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g-TUBE (10)
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Description
10 individually-wrapped g-TUBES for shearing DNA 6kb to 20 kb and one g-TUBE Prep Station Case quantity available;g-TUBE Case (100) 520104
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
g-TUBE (10)
Company
Covaris LLC
Price
Get Pricing
Get Quote for this Product
Catalog Number
520079
Quantity
10
Format
g-TUBE
Type
tubes
Temperature
Ambient
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Covaris LLC
14 Gill St, Unit H
Woburn
,
Massachusetts
01801-1721
United States
Phone
: +1 781-932-3959
Fax: +1 781-932-8705
Email: info@covaris.com
Company Profile
Website:
www.covaris.com
Citations (73)
(21)
Little skate genome provides insights into genetic programs essential for limb-based locomotion
eLife
October 26, 2022
DongAhn Yoo, Junhee Park, Chul Lee, Injun Song, Young Ho Lee, Tery Yun, Hyemin Lee, Adriana Heguy, Jae Yong Han, Jeremy S Dasen,
et al.
little skate genomic DNA (6.5–9 µg in 150 µl volume) was transferred to a
Covaris
g-Tube
(
Covaris
, #
520079
) and sheared in a tabletop centrifuge (Eppendorf, #5424) at 3600 rpm for 30 seconds. After shearing,
(22)
INSERT-seq enables high-resolution mapping of genomically integrated DNA using Nanopore sequencing
Genome Biology
October 25, 2022
Dimitrije IvanÄić, Júlia Mir-Pedrol, Jessica Jaraba-Wallace, Núria Rafel, Avencia Sanchez-Mejias, Marc Güell
(Circulomics, catalogue no. NB-900-001-01), sheared to ~6 kbp fragments using
g-TUBE
™ (
Covaris
, catalogue no.
520079
). WGP primer mix from Nanopore
PCR
Barcoding Kit (SQK-PBK004) was additionally added to the second
PCR
.
(23)
Multi-platform-based characterization of ferroptosis in human colorectal cancer
iScience
August 19, 2022
Yafang Zhong, Wei Zhang, Haiyan Yu, Liewen Lin, Xucan Gao, Jingquan He, Dandan Li, Yumei Chen, Zhipeng Zeng, Yong Xu,
et al.
whole genome sequencing
The DNA was broken into fragments of around 20 kb using a g-TUBETM (
Covaris
,
520079
). Following that, magnetic beads were added (Pacifi Bioscience, 100-317-100). After adding
stem
-loop
(24)
High-throughput method for the hybridisation-based targeted enrichment of long genomic fragments for PacBio third-generation sequencing
NAR Genomics and Bioinformatics
July 13, 2022
Tim Alexander Steiert et al.
water to a total volume of 150 μl and fragmented to a target fragment length of 10 kb with a g-tube (#
520079
,
Covaris
, USA). DNA pre-fragmentation was done by spinning the g-tube two times for 270 s at 1657 g.
(25)
The Complete Genome of the “Flavescence Dorée” Phytoplasma Reveals Characteristics of Low Genome Plasticity
Biology (Basel)
June 23, 2022
Christophe Debonneville, Léa Mandelli, Justine Brodard, Raphaël Groux, David Roquis, Olivier Schumpp
procedure
. 2.3. MinION and
Illumina Sequencing
DNA was fragmented using a g-tube (
Covaris
, Woburn, MA, USA #
520079
) for 1 min at 2000× g. A total of 1 μg of fragmented DNA was used with the
Ligation
Sequencing
kit (Oxford
(26)
DiMeLo-seq: a long-read, single-molecule method for mapping protein–DNA interactions genome wide
Nature Methods
June 1, 2022
Nicolas Altemose, Annie Maslan, Owen K. Smith, Kousik Sundararajan, Rachel R. Brown, Reet Mishra, Angela M. Detweiler, Norma Neff, Karen H. Miga, Aaron F. Straight,
et al.
To fragment the DNA before library preparation, we targeted 20 kb fragments using a
g-Tube
(
Covaris
520079
) with 60-second spins at 4200 rpm. We prepared PacBio libraries for
sequencing
using the SMRTbell® Express
(27)
Long-Read Sequencing Revealed Extragenic and Intragenic Duplications of Exons 56–61 in DMD in an Asymptomatic Male and a DMD Patient
Frontiers in Genetics
May 9, 2022
Ying Bai, Ju Liu, Jinghan Xu, Yue Sun, Jingjing Li, Yong Gao, Lina Liu, Cangcang Jia, Xiangdong Kong, Li Wang
USA) according to the instructions. 3 μg genomic DNA was sheared to 1∼6 kb fragments by a
g-Tube
(#
520079
;
Covaris
, Bankstown, Australia)
centrifugation
. A SMRTbell™ library was prepared and sequenced on R9.4
(28)
Integrated Genomic Analysis Identifies UBTF Tandem Duplications as a Recurrent Lesion in Pediatric Acute Myeloid Leukemia
Blood Cancer Discovery
May 5, 2022
Masayuki Umeda, Jing Ma, Benjamin J. Huang, Kohei Hagiwara, Tamara Westover, Sherif Abdelhamed, Juan M. Barajas, Melvin E. Thomas, Michael P. Walsh, Guangchun Song,
et al.
Monarch HMW DNA Extraction Kit for Cells & Blood
(
T3050L
,
NEB
). About 5 μg HMW DNA was sheared to about 20 Kb using
Covaris
g-TUBE
(
520079
,
Covaris
). Sheared DNA was size selected using
BluePippin
(BLU0001,
Sage Science
) to obtain DNA fragments
(29)
Detection of Structural Variations and Fusion Genes in Breast Cancer Samples Using Third-Generation Sequencing
Frontiers in Cell and Developmental Biology
April 13, 2022
Taobo Hu et al.
manufacturer’s protocol. Briefly, 3 ugs genomic DNA was sheared to around 5–6 kb fragments by a
g-TUBE
(#
520079
,
Covaris
, Woburn, MA, United States)
centrifugation
(15,000 g, 2 min, twice). End-repair and dA-tailing
(30)
Robust Benchmark Structural Variant Calls of An Asian Using the State-of-art Long Fragment Sequencing Technologies
Genomics, Proteomics & Bioinformatics
February 1, 2022
Xiao Du, Lili Li, Fan Liang, Sanyang Liu, Wenxin Zhang, Shuai Sun, Yuhui Sun, Fei Fan, Linying Wang, Xinming Liang,
et al.
was used to detect the integrity of gDNA. A total of 8 μg gDNA was sheared using
g-TUBE
(Catalog No.
520079
,
Covaris
, Woburn, MA) and concentrated with the AMPure PB magnetic beads.
Library construction
and
(31)
Distinguishing between recruitment and spread of silent chromatin structures in Saccharomyces cerevisiae
eLife
January 24, 2022
Brothers M, Rine J.
combined. DNA was sheared to ~15–20 kb by spinning through a
Covaris
g-TUBE
(
Covaris Inc
, Woburn, MA, Cat #
520079
) at 4200 rpm for 1 min, and repeating 1× with the tube flipped the other way in an Eppendorf Centrifuge
(32)
Alu element in the RNA binding motif protein, X-linked 2 (RBMX2) gene found to be linked to bipolar disorder
PLOS ONE
December 16, 2021
Pia Laine et al.
from each of the 16 samples were sheared to an average of 10Kb fragments using g-TubeTM by Covaris (PN
520079
,
Covaris
, MA, USA). For each DNA sample, a library was made using the KAPA HyperPrep Kit® for Illumina
(33)
Multi-species host range of staphylococcal phages isolated from wastewater
Nature Communications
December 1, 2021
Pauline C. Göller, Tabea Elsener, Dominic Lorgé, Natasa Radulovic, Viona Bernardi, Annika Naumann, Nesrine Amri, Ekaterina Khatchatourova, Felipe Hernandes Coutinho, Martin J. Loessner,
et al.
mechanically sheared to the average size distribution of 8–10 kb, using a Covaris gTube (
Covaris
p/n
520079
).
Multiplex
libraries were prepared using the SMRTBell™ Barcoded Adapter Complete Prep Kit–96, following
(34)
LeafGo: Leaf to Genome, a quick workflow to produce high-quality de novo plant genomes using long-read sequencing technology
Genome Biology
December 1, 2021
Patrick Driguez, Salim Bougouffa, Karen Carty, Alexander Putra, Kamel Jabbari, Muppala Reddy, Richard Soppe, Ming Sin Cheung, Yoshinori Fukasawa, Luca Ermini
diluted in EB and was sheared using
g-TUBE
or Megaruptor 2. Samples were loaded into g-TUBEs (
Covaris
,
520079
) and sheared with an Eppendorf 5424 (Eppendorf, Hamburg, Germany) for 2 min each spin. A repeat spin
(35)
Applications and potentials of nanopore sequencing in the (epi)genome and (epi)transcriptome era
Innovation (Camb)
November 28, 2021
Shangqian Xie, Amy Wing-Sze Leung, Zhenxian Zheng, Dake Zhang, Chuanle Xiao, Ruibang Luo, Ming Luo, Shoudong Zhang
modifications, it is better to fragment genomic DNA to 8 kb with
g-Tube
(
Covaris
, Woburn, MA) (Cat#
520079
), so that you can retrieve as much data as possible for downstream analysis. Readers can find more related
(36)
Functional meta-omics provide critical insights into long- and short-read assemblies
Briefings in Bioinformatics
November 5, 2021
Valentina Galata, Susheel Bhanu Busi, Benoît Josef Kunath, Laura de Nies, Magdalena Calusinska, Rashi Halder, Patrick May, Paul Wilmes, Cédric Christian Laczny
size selected using AMpure beads for longer fragments. The DNA was sheared using a G-tube (cat. no.
520079
,
Covaris
) aiming for 8 kb average size according to the protocol provided by the manufacturer. Library
(37)
Coping with darkness: The adaptive response of marine picocyanobacteria to repeated light energy deprivation
Limnology and Oceanography
September 1, 2021
Allison Coe et al.
(10 mM TrisHCl, 1 mM EDTA, pH 8.5) and fragmenting to 10–12 kb lengths using a gTube (
Covaris
Cat#
520079
). Fragmented DNA was concentrated by a 0.45X SPRI bead cleanup (Beckman‐Coulter), eluted in 37 μL EB
(38)
Study of complex structural variations of X-linked deafness-2 based on single-molecule sequencing
Bioscience Reports
June 25, 2021
Yi Jiang et al.
Oxford Nanopore Technologies (ONT, Oxford, U.K.). Briefly, genomic DNA fragments were obtained via
g-TUBE
(#
520079
,
Covaris
, Boston, MA, U.S.A.) and Blue Pippin (Sage Science, Beverly, MA, U.S.A.), and the
Ligation
(39)
Sequence and Structure Characteristics of 22 Deletion Breakpoints in Intron 44 of the DMD Gene Based on Long-Read Sequencing
Frontiers in Genetics
April 30, 2021
Chang Geng, Yuanren Tong, Siwen Zhang, Chao Ling, Xin Wu, Depeng Wang, Yi Dai
prepared as following: 3 μg of genomic DNA per sample was sheared to 1 kb∼6 kb fragments by a
g-Tube
(#
520079
,
Covaris
) and centrifuged (1,5000 × g, 2 min, twice). The DNA was then purified after characterization
(40)
Towards complete and error-free genome assemblies of all vertebrate species
Nature
April 29, 2021
Arang Rhie, Shane A. McCarthy, Olivier Fedrigo, Joana Damas, Giulio Formenti, Sergey Koren, Marcela Uliano-Silva, William Chow, Arkarachai Fungtammasan, Juwan Kim,
et al.
kb fragment size with a MegaRuptor device (Diagenode, Belgium) and fragmented using
Covaris
g-tubes (
520079
) or by needle shearing. PacBio large insert libraries were prepared with either the SMRTbell Template
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g-TUBE (10) from Covaris LLC
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